Fibroblast Antibody (5B5)

Product Discontinued

Fibroblast Antibody (5B5) Summary

• Immunogen: BALB/C mice were injected with human prolyl 4-hydroxylase.
• Marker: Fibroblast Marker
• Specificity: This is specific to beta subunit of prolyl 4-hydroxylase. It reacts with fibroblasts and myoepithelial cells. This does not stain lymphocytes, monocytes, dendritic cells and granulocytes.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:25-1:50
• Application Notes: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. This antibody is not suitable for paraffin tissue sections. It is suitable for frozen tissue sections or cytospins only.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: No buffer
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Fibroblast Antibody (5B5)

• reticular fibroblast

Background

Fibroblasts are found in connective tissue and secrete an extracellular matrix rich in collagen. They migrate to sites of tissue damage to facilitate wound repair and proliferate well in tissue culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblast Antibody (NB110-15846)(1)

Publication using NB110-15846

• Bohm M, Apel M, Lowin T et al. a-MSH modulates cell adhesion and inflammatory responses of synovial fibroblasts from osteoarthritis patients Biochem. Pharmacol. 2016-07-07 [PMID: 27396757]

FAQs for Fibroblast Antibody (NB110-15846). (Showing 1 - 1 of 1 FAQ).

1. I am currently in search of an antibody for staining of human dermal fibroblasts. Can you recommend me an antibody for this? Is there an antibody that specifically stains only fibroblasts and not mesenchymal stem cells?
   • We sell a number of antibodies which are suitable for staining fibroblasts. You can see these at the following links: NB100-64932, NB110-15846 and NB100-1845. NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. NB110-15846 is specific to beta subunit of prolyl 4-hydroxylase. It reacts with fibroblasts and myoepithelial cells, and does not stain lymphocytes, monocytes, dendritic cells or granulocytes. NB100-1845 recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells. Unfortunately, we do not have any data regarding the staining of mesenchymal stem cells with any of these antibodies in our QC lab.