Fibronectin Antibody (2755-8)

Immunohistochemistry-Paraffin: Fibronectin Antibody (2755-8) [NBP2-32851] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (2755-8).

Product Discontinued

Fibronectin Antibody (2755-8) Summary

• Immunogen: T-cell lymphoma biopsy
• Localization: Connective tissue matrix
• Specificity: Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblasts phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • Western Blot 0.5-1ug/ml
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID: 31160441).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS with 0.05% BSA.
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Fibronectin Antibody (2755-8)

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Fibronectin (also known as FN1, or Fibronectin 1) is an extracellular matrix glycoprotein that binds to integrins and has a large role in cell adhesion, migration, differentiation, and growth. It plays a key role in wound healing by helping form blood clots and participates in embryonic development by guiding cell attachment and migration. Fibronectin consists of two monomers linked by disulfide bonds and binds extracellular components such as collagen fibrin and syndecans. Fibronectin has been implicated in carcinoma and tumor development while also limiting tumor cells response to treatment. Novus offers Fibronectin antibodies, ELISA Kits, lysates, and proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibronectin Antibody (NBP2-32851)(2)

Publications using NBP2-32851

• Jayachandran I, Sundararajan S, Venkatesan S et al. Asymmetric dimethylarginine (ADMA) accelerates renal cell fibrosis under high glucose condition through NOX4/ROS/ERK signaling pathway Sci Rep 2020-09-29 [PMID: 32994511] (WB, Rat)
• Blessing WA, Okajima SM, Cubria MB et al. Intraarticular injection of relaxin-2 alleviates shoulder arthrofibrosis Proc. Natl. Acad. Sci. U.S.A. 2019-06-18 [PMID: 31160441] (IF/IHC, Human, Rat)

Bioinformatics

• Gene Symbol: FN1
• Uniprot:
  • P02751()