Fibronectin Antibody (DH1)

• Reactivity: Hu, Rt, Ch, Gp, Rb
• Applications: WB, ELISA, IHC
• Clone: DH1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Fibronectin Antibody (DH1) Summary

• Immunogen: Purified fibronectin from A8387 fibrosarcoma cells
• Marker: Mesenchymal Cells Marker
• Specificity: The antibody is specific to extradomain A (EDA) sequence of a cellular fibronectin and recognizes thus only the cellular fibronectin
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 1% BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Fibronectin Antibody (DH1)

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Integrin beta1 also known as integrin VLA-4 beta chain, fibronectin receptor beta chain, and CD29) is a 130 kD integrin beta chain containing VWFA-like domains. Five isoforms have been reported (beta1A-1D, 1C-2). This Type I membrane protein is a subunit of the heterodimeric receptor for laminin, fibronectin, collagen, fibrinogen, VCAM1, cytotactin, osteopontin, epiligrin, thrombospondin, and vitronectin. Signal transduction through the integrins affects embryogenesis, hemostasis, tissue repair, immune response, and metastatic spread of tumor cells. Integrin beta1 can be upregulated by LPS with soluble CD14 and TNF-alpha; downregulation of integrin beta1 by alpha-melanocyte stimulating hormone and progesterone. Integrin beta1 is modified by phosphorylation and glycosylation. Integrin beta1 partners with integrin alpha subunits alphav, and alpha1 through alpha1.1. The Poly6004 antibody recognizes the N-terminal region of human integrin beta1 and has been shown to be useful for Western blotting. Partners with integrin alpha subunits alphav, alpha1 through alpha11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibronectin Antibody (NBP1-51723)(2)

Publications using NBP1-51723

• Whitehead AJ, Atcha H, Hocker JD et al. AP-1 signaling modulates cardiac fibroblast stress responses Journal of cell science 2023-11-23 [PMID: 37994565] (WB, Human)
• Whitehead AJ, Hocker JD, Ren B, Engler AJ Improved epicardial cardiac fibroblast generation from iPSCs Journal of molecular and cellular cardiology 2021-11-24 [PMID: 34826415] (ICC/IF, WB, Human)

FAQs for Fibronectin Antibody (NBP1-51723). (Showing 1 - 1 of 1 FAQs).

1. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
   • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NBP1-51723, the dilution range is 1:500-1:1000.

Bioinformatics

• Gene Symbol: FN1