FITC Antibody (SPM395) [Alexa Fluor® 647]

• Reactivity: All-NA
• Applications: WB, Flow, Func, ICC/IF, IHC
• Clone: SPM395
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

FITC Antibody (SPM395) [Alexa Fluor® 647] Summary

• Immunogen: Fluorescein isothiocyanate (FITC) conjugated to KLH
• Localization: Depends on the target
• Specificity: This monoclonal antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this monoclonal antibody is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FITC Antibody (SPM395) [Alexa Fluor® 647]

• FITC
• Fluorescein Isothiocyanate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NBP3-08492AF647)(1)

Publication using NBP3-08492AF647

• Zhang AQ, Hostetler A, Chen LE et al. Universal redirection of CAR T cells against solid tumours via membrane-inserted ligands for the CAR Nature biomedical engineering 2023-06-08 [PMID: 37291434]