Recombinant Human FKBP12.6 His Protein

SDS-Page: Recombinant Human FKBP12.6 Protein [NBP1-72505]

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.25 mg/ml

Recombinant Human FKBP12.6 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-108 of Human FKBP12.6
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSMGVEIETI SPGDGRTFPK KGQTCVVHYT GMLQNGKKFD SSRDRNKPFK FRIGKQEVIK GFEEGAAQMS LGQRAKLTCT PDVAYGATGH PGVIPPNATL IFDVELLNLE

• Details of Functionality: Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FKBP1B
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 14.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human FKBP12.6 His Protein

• 12.6 kDa FK506-binding protein
• calstabin 2,12.6 kDa FKBP
• EC 5.2.1.8
• FK506 binding protein 1B, 12.6 kDa
• FK506-binding protein 12.6
• FK506-binding protein 1B (12.6 kD)
• FK506-binding protein 1B
• FKBP12.6
• FKBP-12.6
• FKBP12.6h-FKBP-12
• FKBP1B
• FKBP-1B
• FKBP1L
• FKBP9
• Immunophilin FKBP12.6
• OTK4
• OTK4PPIase FKBP1B
• peptidyl-prolyl cis-trans isomerase FKBP1B
• PKBP1L
• PPIase
• rotamase

Background

FKBP1B, also known as peptidyl-prolyl cis-trans isomerase FKBP1B, is a member of the immunophilin protein family which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 (tacrolimus) and rapamycin (sirolimus). It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. Recombinant human FKBP1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for FKBP12.6 Recombinant Protein (NBP1-72505). (Showing 1 - 3 of 3 FAQ).

1. Could you let us know the MW and concentration of this recombinant? (concentration was not shown on the vial label)
   • The molecular weight for NBP1-72505 is 14.2 kDa (not including the His tag ~26kDa) and the concentration is 0.25mg/mL.
2. I was wondering whether the product is suitable for any kind of assay/experiment since the only use suggested is PAGE?
   • To answer your question regarding the applications FKBP1B Protein (NBP1-72505) can be used for, it has already been tested for its ability to cleave suc-AAFP-pNA but our lab suspects it can be used in other functional assays, although it hasn't been confirmed yet. For untested applications we recommend buying smaller amounts first.
3. For NBP1-72505 (FKBP12.6) for immuno-assay, is the 6HIS ist bound to the C-end or the N-end?
   • The His-tag is linked at the N-t of the protein. I copied the a.a. sequence of FKBP12.6 as follow: "MGSSHHHHHH SSGLVPRGSH RSMGVEIETI SPGDGRTFPK KGQTCVVHYT GMLQNGKKFD SSRDRNKPFK FRIGKQEVIK GFEEGAAQMS LGQRAKLTCT PDVAYGATGH PGVIPPNATL IFDVELLNLE", where the six His within the first 10 a.a. is the His-tag.

Bioinformatics

• Gene Symbol: FKBP1B