Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [Alexa Fluor® 488]

• Reactivity: V-Vi
• Applications: WB, ELISA, Flow, ICC/IF, IHC, B/N, CyTOF-ready, ICC/IF
• Clone: D1-4G2-4-15 (4G2)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [Alexa Fluor® 488] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This recombinant Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) was prepared from Dengue Virus type 2 antigens.
• Specificity: This Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Neutralization
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Dengue Virus, Zika Virus, West Nile Virus, Yellow Fever Virus, Flaviviridae.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [Alexa Fluor® 488]

• Envelope Protein
• Protein E

Background

Flaviviruses are a group of seventy enveloped, positive-sense single stranded RNA viruses belonging to the Flaviviridae family (1-5). Flaviviruses are typically transmitted through arthropods, like mosquitoes and ticks, and can infect humans causing outbreaks and serious illness or death worldwide (1-5). These well-known viral pathogens include dengue virus (DENV), West Nile virus (WNV), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV), Yellow Fever virus (YFV), and Zika virus (ZIKV) (1-5). Flaviviruses consist of ~10.7 kb RNA genome with one open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) (1, 2, 6). The ORF encodes for a polyprotein that is made up of three structural proteins, the capsid (C), premembrane (prM), and envelope (E) proteins, and seven non-structural (NS) proteins (NS1, NS2A/B, NS3, NS4A, 2K, NS4B, and NS5) (1, 2, 5, 6).

The Flavivirus group antigen antibody binds to the fusion loop at the distal end of domain II (EDII) of protein E. Flavivirus E is a glycoprotein that is the main antigen for membrane fusion between virus and host and entry into the cell, as well as inducing protective immunity by stimulating neutralizing antibodies (2, 4, 6). The E protein is 495 amino acids (aa) in length and has a theoretical molecular weight between 55 - 72 kDa, which varies based on glycosylation sites (2). Flavivirus E protein contains three domains (EDI, EDII, and EDIII) followed by a stem that contents to the helix-transmembrane domains (TMDs, TM1 and TM2) (2, 4, 6). The EDII contains an S-S bridge at its distal end and functions as a fusion loop (FL) for virus-mediated membrane fusion and antibody binding (2). Given the important role of Flavivirus protein E in host cell infection and membrane fusion, it has become a target for flavivirus disease prevention and treatment (2, 5).

Alternative names for Flavivirus group antigen include Envelope Protein and Protein E.

References

1. Ngono, A. E., & Shresta, S. (2018). Immune Response to Dengue and Zika. Annual review of immunology. https://doi.org/10.1146/annurev-immunol-042617-053142

2. Rey, F. A., Stiasny, K., Vaney, M. C., Dellarole, M., & Heinz, F. X. (2018). The bright and the dark side of human antibody responses to flaviviruses: lessons for vaccine design. EMBO reports. https://doi.org/10.15252/embr.201745302

3. Zhang, X., Jia, R., Shen, H., Wang, M., Yin, Z., & Cheng, A. (2017). Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections. Viruses. https://doi.org/10.3390/v9110338

4. Laureti, M., Narayanan, D., Rodriguez-Andres, J., Fazakerley, J. K., & Kedzierski, L. (2018). Flavivirus Receptors: Diversity, Identity, and Cell Entry. Frontiers in immunology. https://doi.org/10.3389/fimmu.2018.02180

5. Boldescu, V., Behnam, M., Vasilakis, N., & Klein, C. D. (2017). Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond. Nature reviews. Drug discovery. https://doi.org/10.1038/nrd.2017.33

6. Crill, W. D., & Chang, G. J. (2004). Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes. Journal of virology, 78(24), 13975-13986. https://doi.org/10.1128/JVI.78.24.13975-13986.2004

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flavivirus group antigen Antibody (NBP2-52709AF488)(1)

Publication using NBP2-52709AF488

• Jeong GU, Lee S, Kim DY et al. Zika Virus Infection Induces Interleukin-1beta-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model Journal of virology 2023-05-16 [PMID: 37191498] (FLOW)

FAQs for Flavivirus group antigen Antibody (NBP2-52709AF488). (Showing 1 - 2 of 2 FAQ).

1. What is the recommended concentration to start titration of conjugated Flavivirus group antigen antibodies NBP2-52709PE and NBP2-52709AF488?
   • The recommended dilutions for both of these conjugates can be found on the unconjugated antibody web page under the Datasheet tab in the "Applications/Dilutions" section.   Any papers referencing this antibody (including conjugates) can also be found on the unconjugated antibody page. For flow cytometry, we recommend a dilution of 1:10-1:1000, but the optimal dilution should be determined experimentally.
2. We are interested in ordering the Flavivirus group antigen [D1-4G2-4-15 (4G2)] monoclonal antibody with the Mouse IgG2a isotype (catalog number NBP2-52709). We are aware that the  hybridoma D1-4G2-4-15 (4G2)  produces both IgG2 and IgG1 antibodies. Because of the isotype specifictiy of the application, we would like to confirm that the isotype is purely IgG2a, as stated on the product information page, and not a mix.
   • The two different hybridoma products differ by the host and catalog numbers (NBP2-52709, NBP2-52666), and are not mixed. So if you are looking to get a Mouse IgG2A to your secondary, you can please take a look at NBP2-52709 and see if it is suitable for you.