Recombinant Porcine Flt-3 Ligand/FLT3L Animal-Free Protein

SDS-Page: Recombinant Porcine Flt-3 Ligand Animal-Free Protein [NBP2-76295] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Porcine Flt-3 Ligand Animal-Free Protein [NBP2-76295] - Flt-3 Ligand induced proliferation of OCI-AML5 cells.

Product Discontinued

Recombinant Porcine Flt-3 Ligand/FLT3L Animal-Free Protein Summary

• Description: For prolonged storage, dilute to working aliquots.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: Flt-3 Ligand is a monomer, 17.3 kDa (155 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: OCI-AML5 cell proliferation, >2.0 x 10^5 units/mg (ED50 acceptance criteria are <5 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FLT3LG
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 17.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Alternate Names for Recombinant Porcine Flt-3 Ligand/FLT3L Animal-Free Protein

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FLT3LG