FPRP/PTGFRN Antibody [Biotin]

3T3‑L1 mouse embryonic fibroblast adipose‑like cell line was stained with Sheep Anti-Mouse FPRP Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF4495, filled histogram) or isotype control antibody ???, followed by Streptavidin-Allophycocyanin (Catalog # F0050).

• Reactivity: Mu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

FPRP/PTGFRN Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse FPRP
  Arg22-Pro832
  Accession # NP_035327
• Specificity: Detects mouse FPRP in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Ptgfrn
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FPRP/PTGFRN Antibody [Biotin]

• CD315
• CD9P-1
• FPRP
• PTGFRN

Background

FPRP (F_{2 alpha} Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha}  receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ptgfrn
• Uniprot:
  • NP_035327()