FTO Antibody (5-2H10) - Azide Free

Western Blot: FTO Antibody (5-2H10) [NBP2-29512] - Western blot of rat testes lysate showing specific immunolabeling of the ~ 58k Fto protein.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Eq, Pm, Sh
• Applications: WB, ICC/IF
• Clone: 5-2H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free

FTO Antibody (5-2H10) - Azide Free Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # Q9C0B1
• Specificity: Specific for the ~58 kDa FTO protein in Western blots of rat testes lysate. The antibody has also been used for immunocytochemistry with neuronal progenitor cells.
• Predicted Species: Bovine (100%), Canine (100%), Sheep (100%), Primate (100%), Equine (100%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FTO
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: The antibody has also been used for immunocytochemistry with neuronal progenitor cells.
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for FTO Antibody (5-2H10) - Azide Free

• Alpha-ketoglutarate-dependent dioxygenase FTO
• EC 1.14.11.-
• fat mass and obesity associated
• Fat mass and obesity-associated protein
• FTO
• KIAA1752protein fto
• MGC5149

Background

The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for FTO Antibody (NBP2-29512). (Showing 1 - 1 of 1 FAQ).

1. Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?
   • With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.

Bioinformatics

• Gene Symbol: FTO
• Entrez:
  • 79068(Human)
• Uniprot:
  • Q9C0B1(Human)