FZR1/CDH1 Antibody (DH01 (DCS-266))

Western Blot: FZR1/CDH1 Antibody (DH01 (DCS-266)) [NBP2-22103] - Analysis of Cdh1 on LS174T Cells.

Immunocytochemistry/Immunofluorescence: FZR1/CDH1 Antibody (DH01 (DCS-266)) [NBP2-22103] - Analysis of CD324/E Cadherin (green) showing staining in the in the cytoplasm, membrane of HT-29 cells (right) compared to a negative control without primary antibody (left).

Product Discontinued

FZR1/CDH1 Antibody (DH01 (DCS-266)) Summary

• Immunogen: Recombinant human cdh1 protein
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FZR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunoprecipitation 2 ug/mg
  • Western Blot 1 - 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for FZR1/CDH1 Antibody (DH01 (DCS-266))

• anti-CDC20-like protein 1 antibody
• anti-CDH1 antibody
• anti-Cdh1/Hct1 homolog antibody
• anti-Fizzy-related protein homolog antibody
• anti-FYR antibody
• anti-Fzr antibody
• anti-FZR1 antibody
• anti-hCDH1 antibody
• CDC20C
• CDH1
• Cdh1/Hct1 homolog
• CDH1CDC20-like 1b
• fizzy/cell division cycle 20 related 1 (Drosophila)
• FYR
• Fzr
• FZR2
• FZRCDC20-like protein 1
• hCDH1
• HCDHFYR
• KIAA1242
• KIAA1242fizzy-related protein homolog

Background

FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. FZR1 associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FZR1
• Uniprot:
  • Q9UM11(Human)