G418 Inhibitor

G418 Inhibitor [NBP2-33053]

G418 Inhibitor [NBP2-33053]

• Concentration: Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Applications/Dilutions

• Application Notes: Resistance to G418 is conferred by the neo gene from transposon Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3' II. This protein inactivates G418 by covalently modifying its amino or hydroxyl functions therefore inhibiting the antibiotic-ribosome interaction.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: H2O
• Concentration: Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Background

G418 is used for the selection and maintenance of eukaryotic cells expressing the neo gene1. G418 is an aminoglycoside antibiotic similar in structure to gentamycin B1, produced by Micromonospora rhodorangea. Unlike gentamycin, G418 blocks polypeptide synthesis in eukaryotic cells by binding irreversibly to 80S ribosomes and therefore disrupting their proofreading capability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.