GABA Receptor Epsilon Antibody (S413-67)

Western Blot: GABA Receptor Epsilon Antibody (S413-67) [NBP2-59323] - Analysis of Rat Brain showing detection of ~60 kDa GABA A Receptor Epsilon protein using Mouse Anti-GABA A Receptor Epsilon Monoclonal Antibody, Clone S413-67. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 ug . Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Epsilon Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.

Immunocytochemistry/Immunofluorescence: GABA Receptor Epsilon Antibody (S413-67) [NBP2-59323] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Epsilon Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X.

Product Discontinued

GABA Receptor Epsilon Antibody (S413-67) Summary

• Immunogen: Fusion protein amino acids 27-253 (Extracellular N-terminus) of human GABA A Receptor Epsilon
• Localization: Cell Junction , Synapse , Postsynaptic Cell Membrane , Cell Membrane
• Specificity: Detects 60kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABRE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Purity: Protein G purified

Alternate Names for GABA Receptor Epsilon Antibody (S413-67)

• GABA A R epsilon
• GABA(A) receptor subunit epsilon
• GABAAR epsilon
• GABAARe
• gamma-aminobutyric acid (GABA) A receptor, epsilon
• gamma-aminobutyric acid receptor subunit epsilon

Background

The product of the GABA Receptor Epsilon gene belongs to the ligand-gated ionic channel (TC 1.A.9) family. It encodes the gamma-aminobutyricacid (GABA) A receptor which is a multisubunit chloride channel that mediates the fastest inhibitory synaptictransmission in the central nervous system. This gene encodes an epsilon subunit. It is mapped to chromosome Xq28 in acluster comprised of genes encoding alpha 3, beta 4 and theta subunits of the same receptor. Alternatively splicedtranscript variants have been identified, but only one is thought to encode a protein. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABRE