Galectin-3 Antibody (A3A12) - BSA Free

Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. T98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue). Confocal immunofluorescence using DyLight 488 conjugated galectin-3 antibody. This image was submitted via customer Review. Image using the DyLight 488 form of this antibody.

Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control in the absence of primary antibody (left).

Flow Cytometry: Galectin-3 Antibody (A3A12) [NB300-538] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HT-29 cells with Galectin-3 (A3A12) antibody NB300-538AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells.

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of rat olfactory bulb

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of MCF-7 cells.

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of HeLa cells.

Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control in the absence of primary antibody (left).

Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human ovarian carcinoma (right) compared with a negative control in the absence of primary antibody (left).

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: A3A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Galectin-3 Antibody (A3A12) - BSA Free Summary

• Immunogen: Full length recombinant human galectin-3.
• Epitope: The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.
• Specificity: Galectin 3 (A3A12)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGALS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 6 ug/ml
  • Immunohistochemistry-Paraffin 6 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 19744479). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Galectin-3 Antibody (A3A12) - BSA Free

• AGE-R3
• Carbohydrate-binding protein 35
• CBP 35,35 kDa lectin
• CBP35
• GAL3
• Gal-3
• galactin-3
• Galactose-specific lectin 3
• Galactoside-binding protein
• GALBPCBP35
• Galectin3
• Galectin-3
• GALIG
• IgE-binding protein
• L29
• L31
• L-31
• Laminin-binding protein
• Lectin L-29
• lectin, galactoside-binding, soluble, 3
• LGALS2
• LGALS3
• Mac-2 antigen
• Mac-2
• MAC2GAL3

Background

This antibody has been shown to potentiate the binding of Galectin 3 to IgG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-3 Antibody (NB300-538)(5)

Publications using NB300-538

• Staels B, Rubenstrunk A, Noel B et al. Hepato-protective effects of the dual PPARalpha/delta agonist GFT505 in rodent models of NAFLD/NASH. Hepatology 2013-05-22 [PMID: 23703580] (IHC-P, Mouse)
• Ozbek M, Oztop M Expression Patterns of Galectin-1 and Galectin-3 in Hibernating Anatolian Ground Squirrel (Spermophilus Xanthoprymnus) Gastrointestinal Tract Kocatepe Veterinary Journal 2020-02-23
• Sun YC, Hung KF, Li TY Et al. Transmembrane Mucin 1 Blocks Fluorescein Ingress to Corneal Epithelium Invest Ophthalmol Vis Sci 2022-02-25 [PMID: 35212722] (FLOW, IHC-Fr, Human, Rabbit)
  
  Details:
  Citation using the Biotin version of this antibody.
• Khan N, Halcrow PW, Afghah Z et al. HIV-1 Tat endocytosis and retention in endolysosomes affects HIV-1 Tat-induced LTR transactivation in astrocytes FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-03-01 [PMID: 35113458]
• Liu FT, Hsu DK, Zuberi RI, Hill PN, Shenhav A, Kuwabara I, Chen SS. Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains. This antibody has been used in: Biochemistry, 35: 6073-6079. 1996-01-01 [PMID: 8634249]

Bioinformatics

• Gene Symbol: LGALS3
• Entrez:
  • 3958(Human)
  • 16854(Mouse)
  • 83781(Rat)
• Uniprot:
  • P17931(Mouse)