gamma Tubulin Antibody (TU-32) - BSA Free

Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1alpha, TFAM and SIRT3 in H1299, H1299r, P31 and P31r cell lysates as determined by immunoblot analysis. Whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 and P31r cells. Proteins (90 ug) were resolved in 10% SDS-PAGE gels and transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1alpha, TFAM and SIRT3 or the loading control gamma-tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1alpha, (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885) licensed under a CC-BY license.

Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1alpha, TFAM and SIRT3 in H1299, H1299r, P31 and P31r cell lysates as determined by immunoblot analysis. Whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 and P31r cells. Proteins (90 ug) were resolved in 10% SDS-PAGE gels and transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1alpha, TFAM and SIRT3 or the loading control gamma-tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1alpha, (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885) licensed under a CC-BY license.

Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Analysis of gamma-Tubulin in porcine brain lysate using NBP1-90616.

Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.

Western Blot: gamma Tubulin Antibody (TU-32) - BSA Free [NB110-90616] - Comparative mitochondrial abundance as measured by citrate synthase activity, abundance of VDAC1 & cytochrome oxidase subunit 4(A) Citrate synthase enzyme activity in the H1299 & H1299r cells. (B) Citrate synthase enzyme activity in the P31 & P31r cells. Data are expressed as mean ± SEM for three separate experiments. Statistical analysis was carried out using the student t-test. *** = p< 0.001, ** = p<0.01. For immunoblots, whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 & P31r cells. Proteins (90 μg) were resolved in 10% SDS-PAGE gels & transferred to a PVDF membrane. Blots were probed for either VDAC1, cytochrome c oxidase subunit 4 or the loading control gamma -tubulin. (C) Representative blot of VDAC1 protein expression from three independent experiments. (D) Representative blot of cytochrome c oxidase subunit 4 protein expression from three independent experiments. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Po, An, Pl, Pr
• Applications: WB, ICC/IF
• Clone: TU-32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

gamma Tubulin Antibody (TU-32) - BSA Free Summary

• Immunogen: human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
• Epitope: The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
• Marker: Centrosome Marker
• Specificity: The TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1 ug/ml
• Application Notes: Immunocytochemistry: methanol/acetone fixation required
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 29212260).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for gamma Tubulin Antibody (TU-32) - BSA Free

• gamma Tubulin
• Gamma-1-tubulin
• Gamma-tubulin complex component 1
• GCP-1
• TUBG
• TUBG1
• TUBGCP1
• tubulin gamma-1 chain
• tubulin, gamma 1
• tubulin, gamma polypeptide

Background

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gamma Tubulin Antibody (NB110-90616)(3)

Publications using NB110-90616

• Noone J, Rooney MF, Karavyraki M et al. Cancer-Cachexia-Induced Human Skeletal Muscle Myotube Degeneration Is Prevented via Cannabinoid Receptor 2 Agonism In Vitro Pharmaceuticals (Basel, Switzerland) 2023-11-08 [PMID: 38004445] (WB)
• Geoghegan F, Buckland RJ, Rogers E et al. Bioenergetics of acquired cisplatin resistant H1299 non-small cell lung cancer and P31 mesothelioma cells Oncotarget. 2017-10-16 [PMID: 29212260] (WB, Human)
• Geoghegan F, Chadderton N, Farrar GJ et al. Direct effects of phenformin on metabolism/bioenergetics and viability of SH-SY5Y neuroblastoma cells Oncol Lett. 2017-09-13 [PMID: 29113281] (WB, Human)

Bioinformatics

• Gene Symbol: TUBG1
• Entrez:
  • 7283(Human)
  • 103733(Mouse)
  • 252921(Rat)
• Uniprot:
  • P23258(Human)