GDF-11/BMP-11 Antibody [Unconjugated]

GDF‑11/BMP‑11 was detected in immersion fixed frozen sections of mouse embryo (11 d.p.c.) using Goat Anti-Human/Mouse GDF‑11/BMP‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1958) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

GDF-11/BMP-11 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GDF‑11/BMP‑11
  Asn299-Ser407
  Accession # O95390
• Specificity: Detects human and mouse GDF‑11/BMP‑11 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human/mouse/rat GDF-8/Myostatin and less than 1% cross-reactivity with recombinant human BMP-6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GDF11
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-11/BMP-11 Antibody [Unconjugated]

• BMP11
• BMP-11
• BMP-11BMP11Bone morphogenetic protein 11
• GDF11
• GDF-11
• growth differentiation factor 11
• growth/differentiation factor 11

Background

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide which contains a signal sequence for secretion and an RXXR proteolytic processing site to yield a 109 aa residue carboxy-terminal mature protein (1). Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99‑100% identical. GDF-11 and GDF-8 share 90% amino acid sequence identity within the mature protein. As detected by in situ hybridization, GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1, 2). Likewise, a targeted deletion of GDF-11 in mice results in a spectrum of abnormalities including palatal malformation, vertebral defects, elongated trunks with a reduced or absent tail, missing or malformed kidneys, and an increased number of neurons in the olfactory epithelium (2-5). An intriguing finding in the knockout mice was that the trunk elongation was due to an increase in the number of thoracic vertebrae (4). This implicates GDF-11 as the first secreted factor to influence the specification of segmental identity in vertebrates (3). In fact, GDF-11 does regulate expression of segmental transcription factors, the Hox genes (6). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (7). Despite the strong expression in the limb throughout development, no limb abnormalities were found in the knockout mice. However, in vitro micromass studies indicate that GDF-11 inhibits myogenic and chondrogenic cell differentiation and may impact formation and development of the limb skeleton (6).

1. Gamer, L.W. et al. (1999) Dev. Biol. 208: 222.
2. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
3. Gad, J.M. and P.P.L. Tam (1999) Curr. Biol. 9:R783.  
4. McPherron, A.C. et al. (1999) Nat. Genet. 22:260.
5. Esquela, A.F. and S.J. Lee (2003) Dev. Biol. 257:356.
6. Gamer, L.W. et al. (2001) Dev. Biol. 229:407.
7. Oh, S.P. et al. (2002) Genes & Dev. 16:274.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GDF-11/BMP-11 Antibody (AF1958)(1)

Publication using AF1958

• XJ Zhang, H Tan, ZF Shi, N Li, Y Jia, Z Hao Growth differentiation factor 11 is involved in isoproterenol?induced heart failure Mol Med Rep, 2019-03-22;0(0):. 2019-03-22 [PMID: 30942402] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: GDF11
• Uniprot:
  • O95390()