Recombinant Human GDF-15 Protein

SDS-Page: Recombinant Human GDF-15 Protein [NBP2-76204] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Product Discontinued

Recombinant Human GDF-15 Protein Summary

• Description: For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: GDF-15 is a dimer, 12.4/24.8 kDa (113/226 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GDF15
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 24.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Alternate Names for Recombinant Human GDF-15 Protein

• GDF15
• GDF-15
• growth differentiation factor 15
• growth/differentiation factor 15
• Macrophage inhibitory cytokine 1
• MIC-1
• MIC-1NSAID-activated gene 1 protein
• MIC1Prostate differentiation factor
• NAG-1
• NAG-1NSAID-regulated gene 1 protein
• NSAID (nonsteroidal inflammatory drug)-activated protein 1
• PDF
• PDFGDF-15
• PLAB
• PLABNRG-1
• Placental bone morphogenetic protein
• Placental TGF-beta
• PTGF-beta
• PTGFBPTGF-beta

Background

Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.

GDF15 antibodies are useful for studies on inflamation and immune system research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for GDF-15 Recombinant Protein (NBP2-76204)(3)

Publications using NBP2-76204

• Moura P, Mortera-Blanco T, Hofman I et al. Erythroid differentiation intensifies RNA mis-splicing inSF3B1-mutant myelodysplastic syndromes with ring sideroblasts bioRxiv 2023-04-12
  
  Details:
  CD34+ HSPCs from 3 NBM donors and 5 SF3B1mt 362 MDS-RS patients were treated with RPMI plus 100 ng/mL recombinant GDF15.
• Moura PL, Mortera Blanco T, Hofman IJ et al. Erythroid differentiation enhances RNA mis-splicing in SF3B1-mutant myelodysplastic syndromes with ring sideroblasts Cancer research 2023-11-03 [PMID: 37921711]
• Liao J, Gan Y, Peng M et al. GDF15 alleviates the progression of benign tracheobronchial stenosis by inhibiting epithelial-mesenchymal transition and inactivating fibroblasts Experimental cell research 2022-12-15 [PMID: 36336027] (In Vivo, Rat)

Bioinformatics

• Gene Symbol: GDF15