GFP Antibody

Product Discontinued

GFP Antibody Summary

• Immunogen: EGFP, a native full-length protein
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-3 ug/ml
  • Immunoprecipitation 10-20ug/Sample
  • Western Blot 0.5-1.5 ug/ml
• Application Notes: Western Blotting-Positive control: transfected cells-Negative control: non-transfected cells Immunocytochemistry-Positive control:transfected cells-Negative control:non-transfected cells

Reactivity Notes

The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in Monkey and Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GFP Antibody

• eGFP
• Enhanced Green Fluorescent Protein
• GFP
• GFPuv
• green fluorescent protein (gfp)
• Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for GFP Antibody (NBP2-22220) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Other.

reviewed by: Verified Customer ICC Other 01/16/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: GFP expressing pig TM cells
• Species: Other

Comments

• Comments: Pig TM cells express GFP
  Plenti CMV EGFP DEST plasmid and lenti packaging plasmids were transfected to HEK 293 cells, after two days lenti virus was produced and transduced to pig TM cells for 2-3 days.

reviewed by: Verified Customer WB Other 01/04/2017

Summary

• Application: Western Blot
• Sample Tested: Pig TM cells transduced with GFP
• Species: Other

Comments

• Comments: WB blot showing detection of GFP (red color) using GFP antibody (NBP2-22220) at 1:2000 dilution and the cell lysates from pig TM cells transduced with lenti virus carrying GFP

reviewed by: Verified Customer WB Other 01/22/2015

Summary

• Application: Western Blot
• Sample Tested: Cos-7 whole cell lysate
• Species: Other
• Lot: 517777

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