Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

ELISA: Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric) [NBP2-82203] - Samples were spiked with high concentrations of Human Glucocorticoid Receptor alpha and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric) [NBP2-82203] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Glucocorticoid Receptor alpha in samples. No Significant cross-reactivity or interference between Human Glucocorticoid Receptor alpha and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 6
• Intra-Assay: %CV < 5
• Spike Recovery: average = 92% for serum; average = 91% for EDTA plasma; average = 95% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NR3C1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Glucocorticoid Receptor alpha ELISA Kit (Colorimetric)

• GCR
• glucocorticoid receptor
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the Glucocorticoid Receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform translocates from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR alpha has a greater affinity for GR response elements (GREs) than GR beta only when in the ligand bound state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: NR3C1