![Western Blot: Glucose Oxidase Antibody [NBP2-44186] - Analysis under reducing and non-reducing conditions.](http://images.novusbio.com/fullsize/Glucose-Oxidase-Antibody-Western-Blot-NBP2-44186-img0002.jpg "Western Blot: Glucose Oxidase Antibody [NBP2-44186] - Analysis under reducing and non-reducing conditions.")
| Reactivity | FuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | This Anti-Glucose Oxidase Antibody product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger] Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Glucose Oxidase [Aspergillus niger] (Uniprot: P13006) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This product has been tested in western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Multi-step |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Uniprot |
|
![Western Blot: Glucose Oxidase Antibody [NBP2-44186] - Analysis under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti Glucose Oxidase antibody.](http://images.novusbio.com/fullsize/Glucose Oxidase Antibody-Western Blot-NBP2-44186-img0003.jpg "Western Blot: Glucose Oxidase Antibody [NBP2-44186] - Analysis under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti Glucose Oxidase antibody.")
