Glutamine Synthetase Antibody (3B6)

Western Blot: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - Analysis of GLUL expression in transfected 293T cell line by GLUL monoclonal antibody (M02), clone 3B6.Lane 1: GLUL transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.

Western Blot: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - GLUL monoclonal antibody (M02), clone 3B6. Analysis of GLUL expression in PC-12.

Western Blot: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - GLUL monoclonal antibody (M02), clone 3B6. Analysis of GLUL expression in HepG2.

Western Blot: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - GLUL monoclonal antibody (M02), clone 3B6. Analysis of GLUL expression in Raw 264.7.

Western Blot: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - GLUL monoclonal antibody (M02), clone 3B6 Analysis of GLUL expression in Jurkat.

Sandwich ELISA: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - Detection limit for recombinant GST tagged GLUL is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

Glutamine Synthetase Antibody (3B6) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GLUL (NP_002056, 274 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN
• Specificity: GLUL - glutamate-ammonia ligase (glutamine synthetase)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLUL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glutamine Synthetase Antibody (3B6)

• cell proliferation-inducing protein 59
• EC 4.1.1.15
• EC 6.3.1.2
• GLNSPIG59
• Glutamate decarboxylase
• glutamate-ammonia ligase (glutamine synthase)
• glutamate-ammonia ligase
• Glutamate--ammonia ligase
• glutamine synthase
• glutamine synthetase
• GS
• PIG43
• proliferation-inducing protein 43

Background

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutamine Synthetase Antibody (H00002752-M02)(3)

Publications using H00002752-M02

• Polletta L, Vernucci E, Carnevale I et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy 2015-01-01 [PMID: 25700560]
• Gross MI, Demo SD, Dennison JB et al. Antitumor Activity of the Glutaminase Inhibitor CB-839 in Triple-Negative Breast Cancer. Mol. Cancer Ther. 2014-02-13 [PMID: 24523301] (WB, Human)
• Herbert MK, Kuiperij HB, Verbeek MM. Optimisation of the quantification of glutamine synthetase and myelin basic protein in cerebrospinal fluid by a combined acidification and neutralisation protocol. J Immunol Methods. 2012-04-19 [PMID: 22542401]

Bioinformatics

• Gene Symbol: GLUL
• Entrez:
  • 2752(Human)
• OMIM:
  • 138290(Human)
• Uniprot:
  • NP_002056(Human)