Glutamine Synthetase Activity Assay Kit (Colorimetric) Summary
Description |
Detection instrument: Microplate reader (330 - 350 nm, optimum wavelength: 340 nm) Assay time: 15 min |
Standard Curve Range |
8.84 - 321.05 U/L |
Sensitivity |
8.84 U/L |
Inter-Assay |
%CV < 5.8 |
Intra-Assay |
%CV < 2.5 |
Spike Recovery |
Average recovery rate: 104% |
Kit Type |
Activity Assay Kit (Colorimetric) |
Gene |
GLUL |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Plate Sealer
- Reagent 1: Extracting Solution
- Reagent 2: Buffer Solution
- Reagent 3: Substrate A
- Reagent 4: Substrate B
- Reagent 5: Chromogenic Agent
- Reagent 6: Standard
- UV Microplate
|
Alternate Names for Glutamine Synthetase Activity Assay Kit (Colorimetric)
Background
Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.
Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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