Glutamine Synthetase Activity Assay Kit (Colorimetric)

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Glutamine Synthetase Activity Assay Kit (Colorimetric) [NBP3-24495] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plant tissue and animal tissue
Standard Curve Range
8.84 - 321.05 U/L
Sensitivity
8.84 U/L

Order Details

Glutamine Synthetase Activity Assay Kit (Colorimetric) Summary

Description
Detection instrument: Microplate reader (330 - 350 nm, optimum wavelength: 340 nm)
Assay time: 15 min
Standard Curve Range
8.84 - 321.05 U/L
Sensitivity
8.84 U/L
Inter-Assay
%CV < 5.8
Intra-Assay
%CV < 2.5
Spike Recovery
Average recovery rate: 104%
Kit Type
Activity Assay Kit (Colorimetric)
Gene
GLUL

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Plate Sealer
  2. Reagent 1: Extracting Solution
  3. Reagent 2: Buffer Solution
  4. Reagent 3: Substrate A
  5. Reagent 4: Substrate B
  6. Reagent 5: Chromogenic Agent
  7. Reagent 6: Standard
  8. UV Microplate

Alternate Names for Glutamine Synthetase Activity Assay Kit (Colorimetric)

  • cell proliferation-inducing protein 59
  • EC 4.1.1.15
  • EC 6.3.1.2
  • GLNSPIG59
  • Glutamate decarboxylase
  • glutamate-ammonia ligase (glutamine synthase)
  • glutamate-ammonia ligase
  • Glutamate--ammonia ligase
  • glutamine synthase
  • glutamine synthetase
  • GS
  • PIG43
  • proliferation-inducing protein 43

Background

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GLUL