Glutaminyl-peptide Cyclotransferase/QPCT Antibody

Glutaminyl-peptide Cyclotransferase/QPCT was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Glutaminyl-peptide Cyclotransferase/QPCT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6368) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Glutaminyl-peptide Cyclotransferase/QPCT Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Glutaminyl-peptide Cyclotransferase/QPCT
  Ala33-Leu361
  Accession # Q16769
• Specificity: Detects human Glutaminyl-peptide Cyclotransferase/QPCT in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: QPCT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutaminyl-peptide Cyclotransferase/QPCT Antibody

• EC 2.3.2.5
• GCT
• Glutaminyl cyclase
• glutaminyl-peptide cyclotransferase
• Glutaminyl-tRNA cyclotransferase
• Glutamyl cyclase
• QCEC
• QPCT
• sQC

Background

Glutaminyl-peptide Cyclotransferase, also known as Glutaminyl Cyclase, catalyzes the conversion of N‑terminal L-glutaminyl residues of peptides to pyroglutamyl groups (1). The enzyme is present in the pituitary and adrenal glands, where it is important for the generation of the N‑terminal pyroglutamyl groups of peptide hormones such as neurotensin and thyrotropin-releasing hormone. Glutaminyl Cyclase also catalyzes the conversion of N‑terminal L‑glutamyl residues to pyroglutamyl residues (2). This activity may contribute to the formation of several amyloid-related plaque forming peptides, contributing to Alzheimer’s disease pathology. Glutaminyl Cyclase is also considered to be a diagnostic marker of thyroid tumors (3).

1. Busby, W.H. Jr. et al. (1987) J. Biol. Chem. 262:8532.
2. Schilling, S. et al. (2004) FEBS Lett. 563:191.
3. Griffith, O.L. et al. (2006) J. Clin. Oncol. 24:5043.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: QPCT
• Uniprot:
  • Q16769()