Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein

SDS-Page: Recombinant Human Glutathione Peroxidase 3/GPX3 Protein [NBP1-99090]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-226 of Human Glutathione Peroxidase 3/GPX3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQSRGQEKSK MDCHGGISGT IYEYGALTID GEEYIPFKQY AGKYVLFVNV ASYCGLTGQY IELNALQEEL APFGLVILGF PCNQFGKQEP GENSEILPTL KYVRPGGGFV PNFQLFEKGD VNGEKEQKFY TFLKNSCPPT SELLGTSDRL FWEPMKVHDI RWNFEKFLVG PDGIPIMRWH HRTTVSNVKM DILSYMRRQA ALGVKRK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GPX3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 25.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1 mM DTT, 50 mM Imidazole
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein

• EC 1.11.1
• EC 1.11.1.9
• Extracellular glutathione peroxidase
• glutathione peroxidase 3 (plasma)
• Glutathione Peroxidase 3
• GPX3
• GPx-3
• GPXP
• GPX-P
• GSHPx-3
• GSHPx-P
• Plasma glutathione peroxidase

Background

GPX3, also known as glutathione peroxidase 3, belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. GPX3 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX3(U73C) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GPX3