Glypican 2 Antibody

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Glypican 2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glypican 2
  Pro18-Ser553
  Accession # Q8N158
• Specificity: Detects human Glypican 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant mouse Glypican 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPC2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 2 Antibody

• 2410016G05Rik
• Cerebroglycan
• Glypican 2
• GPC2

Background

The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a lycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1‑GPC6). All glypican core proteins contain an N‑terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35‑63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17‑25% sequence similarity (1‑4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine‑GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).

1. Filmus, J and S.B. Selleck (2001) J. Clin. Invest. 108:497.
2. De Cat, B and G. David (2001) Semin. Cell Dev. Biol. 12:117.
3. Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
4. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
5. Ivins, J.K. et al. (1997) Dev. Biol. 184:320.
6. Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 2 Antibody (AF2304)(2)

Publications using AF2304

• C Brégère, U Fisch, FS Halbeisen, C Schneider, T Dittmar, S Stricker, S Aghlmandi, R Guzman Doublecortin and Glypican-2 concentrations in the cerebrospinal fluid from infants are developmentally downregulated PLoS ONE, 2023-02-17;18(2):e0279343. 2023-02-17 [PMID: 36800341] (ELISA Detection, Human)
• S Lugert, T Kremer, R Jagasia, A Herrmann, S Aigner, C Giachino, I Mendez-Dav, AM Gardier, JP Carralot, H Meisterman, A Augustin, MD Saxe, J Lamerz, G Duran-Pach, A Ducret, V Taylor, DJ David, C Czech Glypican-2 levels in cerebrospinal fluid predict the status of adult hippocampal neurogenesis Sci Rep, 2017-04-25;7(0):46543. 2017-04-25 [PMID: 28440309] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: GPC2
• Uniprot:
  • Q8N158()