Glypican 4 Antibody (4A3)

Product Discontinued

Glypican 4 Antibody (4A3) Summary

• Immunogen: Recombinant human GPC4 (401-529aa) purified from E. coli (NP_001439)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPC4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:250-1:500
  • Western Blot 1:250-1:500
• Application Notes: Use in Flow Cytometry reported in scientific literature (PMID: 23744997)
• Theoretical MW: 62.412 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Glypican 4 Antibody (4A3)

• Glypican 4
• glypican proteoglycan 4
• glypican-4
• GPC4
• K-glypican
• K-glypicandJ900E8.1 (glypican 4)

Background

The glypican (Gpc) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the stage of intiation of neural tube closure, suggesting that glypican-4 is unlikely to play an essential role in convergent extension in the mouse

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 4 Antibody (NBP1-45286)(1)

Publication using NBP1-45286

• Cheong YK, Ngoh ZX, Peh GS et al. Identification of Cell Surface Markers Glypican-4 and CD200 That Differentiate Human Corneal Endothelium From Stromal Fibroblasts. Invest Ophthalmol Vis Sci. 2013-07-08 [PMID: 23744997] (FLOW)

Bioinformatics

• Gene Symbol: GPC4
• Entrez:
  • 2239(Human)
• Uniprot:
  • O75487()