GPR75 Antibody (689705)

GPR75 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human GPR75 Monoclonal Antibody (Catalog # MAB6728) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 689705
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GPR75 Antibody (689705) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPR75
  Accession # O95800
• Specificity: Detects human GPR75 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPR75
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR75 Antibody (689705)

• G protein-coupled receptor 75
• GPR75
• GPRchr2
• MGC132002
• probable G-protein coupled receptor 75
• WI31133
• WI-31133

Background

GPR75 is a 540 amino acid, 7-transmembrane G-protein-coupled glycoprotein receptor mainly expressed in retinal pigment epithelial cells and brain. Polymorphisms in humans have been associated with macular degeneration. GPR75 has been identified as a receptor for CCL5/RANTES in the brain. Collectively, the extracellular domains of human GPR75 share 75% amino acid sequence identity with the corresponding regions of mouse and rat GPR75.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR75
• Uniprot:
  • O95800()