Granzyme B Antibody (GB11) - BSA Free

ELISA: Granzyme B Antibody (GB11) [NB100-64774] - Analysis of human granzyme B using Mouse anti Human granzyme B as a capture reagent and biotinylated Mouse anti Human granzyme B as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm. Human plasma samples are diluted i:2 (green) and 1:4 (blue).

• Reactivity: Hu
• Applications: ELISA, Flow, IHC, IP
• Clone: GB11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Granzyme B Antibody (GB11) - BSA Free Summary

• Immunogen: Purified human Granzyme B
• Specificity: NB100-64774 recognizes the serine protease Granzyme B (GZMB)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GZMB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 2 ug/ml-5 ug/ml
  • Flow Cytometry 1:10-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: For Flow Cytometry: Membrane permeabilisation is required for this application. Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. In ELISA, this product has been used as a capture antibody with NB100-62617 used as the detection antibody.

Reactivity Notes

Predicted cross-reactivities: Chimpanzee, Monkey, Rhesus Monkey

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris buffered saline
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Granzyme B Antibody (GB11) - BSA Free

• C11
• CCPI
• CGL1
• CGL-1
• CSPB
• CSP-B
• CTLA1
• CTLA-1
• CTSGL1
• Fragmentin-2
• Granzyme B
• Granzyme-2
• GranzymeB
• GRB
• GrzB
• GZMB
• HLP
• SECT

Background

Granzyme B is a cysteine protease found in the cytoplasmic lysosome-like granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells and is is required for the induction of target cell lysis. This occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GZMB
• Entrez:
  • 3002(Human)
• Uniprot:
  • P10144()
  • P10144()