Growth Cone Antibody (2G13)

Immunohistochemistry-Paraffin: Growth Cone Antibody (2G13) [NB600-785] - Staining of growth cones of primary olfactory neurons within the olfactory bulb in the developing rat brain using NB 600-785.

• Reactivity: Ch
• Applications: IHC
• Clone: 2G13
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Growth Cone Antibody (2G13) Summary

• Immunogen: Embryonic chick tectal membranes
• Specificity: This is useful as a general reagent for the detection of axonal growth cones in tissue sections and potentially a key marker in neuronal development research.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This antibody can be used in Immunohistochemistry. *Optimal dilutionsshould be determined by the end user. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. This antibody does not work in Flow Cytometry.

Reactivity Notes

Predicted cross-reactivities: Rat, Mouse

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue Culture Supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Background

This antibody recognises an intracellular protein 2G13p which is restricted to growth cones in developing brain and embryonic neurons in culture. The protein 2G13p also appears to be associated with filamentous actin in the growth cones suggesting a role for 2G13p in growth cone motility.This antibody is suitable for use in immunohistological studies of both frozen and paraffin-embedded sections, staining growth cones, including their filopodia and lamellipodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Growth Cone Antibody (NB600-785)(7)

Publications using NB600-785

• Tahtamouni, LH;Alderfer, SA;Kuhn, TB;Minamide, LS;Chanda, S;Ruff, MR;Bamburg, JR; Characterization of a Human Neuronal Culture System for the Study of Cofilin-Actin Rod Pathology Biomedicines 2023-10-31 [PMID: 38001943]
• Toro CA, Hansen J, Siddiq MM et al. The Human ApoE4 Variant Reduces Functional Recovery and Neuronal Sprouting After Incomplete Spinal Cord Injury in Male Mice Frontiers in Cellular Neuroscience 2021-02-18 [PMID: 33679326] (, )
• Bates RC, Stith BJ, Stevens KE. Chronic central administration of valproic acid: Increased pro-survival phospho-proteins and growth cone associated proteins with no behavioral pathology Pharmacol Biochem Behav 2012-08-31 [PMID: 22960225] (IF/IHC, Rat)
• Ryu V, Gallaher Z, Czaja K. Plasticity of nodose ganglion neurons after capsaicin- and vagotomy-induced nerve damage in adult rats. Neuroscience 2010-06-02 [PMID: 20197082] (Rat)
• Espejo, C et al. Time-course expression of CNS inflammatory, neurodegenerative tissue repair markers and metallothioneins during experimental autoimmune encephalomyelitis. Neuroscience 132(4):1135-49. 2005-01-01 [PMID: 16078373]
• Baloui, H et al. Cellular prion protein/laminin receptor: distribution in adult central nervous system and characterization of an isoform associated with a subtype of cortical neurons. Eur. J. Neurosci. 20(10): 2605-2616. 2004-01-01 [PMID: 15548204]
• Stettler O et al. Monoclonal antibody 2G13, a new axonal growth cone marker. J Neurocytol 28:1035-44. 1999-01-01 [PMID: 11054903]

Bioinformatics

• Uniprot:
  • P50890(Chicken)