Growth Hormone R Antibody

Growth Hormone R/GHR was detected in perfusion fixed frozen sections of mouse liver using Mouse Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Growth Hormone R Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Growth Hormone R/GHR
  Thr25-Gln273
  Accession # Q3UP14
• Specificity: Detects mouse Growth Hormone R/GHR in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant rat GHR is observed and approximately 20% cross-reactivity with recombinant human GHR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GHR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Growth Hormone R Antibody

• GH receptor
• GHBP
• GHR
• growth hormone binding protein
• Growth Hormone R
• growth hormone receptor
• serum binding protein
• Somatotropin receptor

Background

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Mouse GHR cDNA encodes a 650 amino acid (aa) residue type I transmembrane protein with a 24 aa signal peptide, a 249 aa extracellular domain, a 24 aa transmembrane domain, and a 353 aa cytoplasmic domain (5). An alternatively spliced secreted isoform of mouse GHR also exists (6). This variant corresponds to the serum GH‑binding protein. Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (7). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8).

1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
3. Clark, R. (1997) Endocr. Rev. 18:157.
4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
5. Smith, W.C. et al. (1989) Mol. Endocrinol. 3:984.
6. Edens, A. et al. (1994) Endocrinol. 135:2802.
7. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
8. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Growth Hormone R Antibody (AF1360)(8)

Publications using AF1360

• Alyssa J. J. Paganoni, Rossella Cannarella, Roberto Oleari, Federica Amoruso, Renata Antal, Marco Ruzza et al. Insulin-like Growth Factor 1, Growth Hormone, and Anti-Müllerian Hormone Receptors Are Differentially Expressed during GnRH Neuron Development International Journal of Molecular Sciences 8/22/2023 [PMID: 37685880]
• Luo, YY;Zeng, X;Zhu, L;Li, C;Xie, J;Dong, Q;Sun, QY;Huang, GN;Li, JY; Growth hormone reduces aneuploidy and improves oocytes quality by JAK2-MAPK3/1 pathway in aged mice Journal of translational medicine 2023-06-29 [PMID: 37386516] (ICC, Mouse)
• T Sawada, D Arai, X Jing, M Miyajima, SJ Frank, K Sakaguchi Molecular interactions of EphA4, growth hormone receptor, Janus kinase 2, and signal transducer and activator of transcription 5B PLoS ONE, 2017-07-07;12(7):e0180785. 2017-07-07 [PMID: 28686668] (Western Blot, Mouse)
• Growth hormone is increased in the lungs and enhances experimental lung metastasis of melanoma in DJ-1 KO mice BMC Cancer, 2016-11-08;16(1):871. 2016-11-08 [PMID: 27825319] (Western Blot, Mouse)
• Corrick , Ryan M, Li , Li, Frank , Stuart J, Messina , Joseph L Hepatic growth hormone resistance after acute injury. Endocrinology, 2013-02-15;154(4):1577-88. 2013-02-15 [PMID: 23417424] (Western Blot, Mouse)
• Johanna L. Barclay, Linda M. Kerr, Leela Arthur, Jennifer E. Rowland, Caroline N. Nelson, Mayumi Ishikawa et al. In Vivo Targeting of the Growth Hormone Receptor (GHR) Box1 Sequence Demonstrates that the GHR Does Not Signal Exclusively through JAK2 Molecular Endocrinology 1/1/2010 [PMID: 19884384]
• Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation. J. Exp. Med., 2009-03-30;206(4):819-31. 2009-03-30 [PMID: 19332876] (ICC, Western Blot, Mouse)
• Anzo M, Cobb LJ, Hwang DL, Mehta H, Said JW, Yakar S, LeRoith D, Cohen P Targeted deletion of hepatic Igf1 in TRAMP mice leads to dramatic alterations in the circulating insulin-like growth factor axis but does not reduce tumor progression. Cancer Res., 2008-05-01;68(9):3342-9. 2008-05-01 [PMID: 18451161] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GHR
• Uniprot:
  • Q3UP14()