GSK-3 alpha/beta [p Tyr216] Antibody (M132)

Western Blot: GSK-3 alpha/beta [p Tyr216] Antibody (M132) [NBP3-23367] - Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3 beta (lanes 1 & 2) or anti-GSK-3 alpha/beta (Y279/Y216) (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: M132
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GSK-3 alpha/beta [p Tyr216] Antibody (M132) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M132 was generated from a phospho-GSK-3 beta (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 216 of human GSK-3 beta. This peptide sequence is also found in GSK-3 alpha (Tyr-279) and is highly conserved in GSK-3 genes in rat and mouse. [UniProt# P49840]
• Modification: p Tyr216
• Specificity: The antibody detects 46/50 kDa proteins corresponding to the apparent molecular mass of GSK-3 alpha/beta on SDS-PAGE immunoblots of pervanadate treated rabbit fibroblasts, as well as treated human SKN-SH and A431 cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSK3A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for GSK-3 alpha/beta [p Tyr216] Antibody (M132)

• DKFZp686D0638
• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 alpha
• glycogen synthase kinase-3 alpha
• GSK-3 alpha
• GSK-3 alpha/beta

Background

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSK3A