H-2Kd Antibody (SF1-1.1) [Biotin]

Product Discontinued

H-2Kd Antibody (SF1-1.1) [Biotin] Summary

• Immunogen: This antibody was generated by immunizing host with BALB/c Mouse cells
• Localization: Nucleated cells of the H-2K^d haplotype
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Mouse d haplotype, Weakly cross-reacts with H-2^k haplotype, Does not cross react with other haplotypes (e.g., b, j, p, q, s, v).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for H-2Kd Antibody (SF1-1.1) [Biotin]

• H2Kd
• H-2Kd
• MHC class I H-2Kd

Background

The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The SF1-1.1 antibody is weakly cross-reactive with H-2k, but does not cross-react with other haplotypes (b, j, p, q, s, v).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.