HB-EGF Antibody [Unconjugated]

Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HB-EGF at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HB-EGF was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8239) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

HB-EGF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse HB-EGF
  Asp63-Leu148
  Accession # Q06186
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HBEGF
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HB-EGF Antibody [Unconjugated]

• diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor)
• Dtr
• DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor)
• Dts
• DTSF
• HBEGF
• HB-EGF
• Hegfl
• heparin-binding EGF-like growth factor
• heparin-binding epidermal growth factor
• proheparin-binding EGF-like growth factor

Background

Heparin-binding EGF-like growth factor, HB-EGF, is a 19-23 kDa glycoprotein, member of the EGF family of mitogens that exists in multiple forms as a result of heterogenous O-glycosylation.  HB-EGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function and promotes smooth muscle cell proliferation. HB-EGF may be involved in macrophage-mediated cellular proliferation. The cDNA for mouse Proheparin-binding EGF-like growth factor encodes a 208 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB‑EGF. Like EGF, TGF-alpha , and AR, HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HB-EGF Antibody (AF8239)(10)

Publications using AF8239

• Yulong Wei, Xiaoyuan Ma, Hao Sun, Tao Gui, Jun Li, Lutian Yao et al. EGFR Signaling Is Required for Maintaining Adult Cartilage Homeostasis and Attenuating Osteoarthritis Progression Journal of Bone and Mineral Research 5/1/2022 [PMID: 35191092]
• L Zhou, W Zhou, AM Joseph, C Chu, GG Putzel, B Fang, F Teng, M Lyu, H Yano, KI Andreasson, E Mekada, G Eberl, GF Sonnenberg Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation Nature Immunology, 2022-01-31;23(2):251-261. 2022-01-31 [PMID: 35102343] (Western Blot, Mouse)
• Yulong Wei, Lijun Luo, Tao Gui, Feifan Yu, Lesan Yan, Lutian Yao et al. Targeting cartilage EGFR pathway for osteoarthritis treatment Science Translational Medicine 1/13/2021 [PMID: 33441426]
• Yulong Wei, Lijun Luo, Tao Gui, Feifan Yu, Lesan Yan, Lutian Yao et al. Targeting cartilage EGFR pathway for osteoarthritis treatment Science Translational Medicine 2021-01-13 [PMID: 33441426] (Western Blot, Mouse)
• Bryan Marsh, Robert Blelloch Single nuclei RNA-seq of mouse placental labyrinth development eLife 11/3/2020 [PMID: 33141023]
• Ian J. Villamagna, Trent N. Gordon, Mark B. Hurtig, Frank Beier, Elizabeth R. Gillies Poly(ester amide) particles for controlled delivery of celecoxib Journal of Biomedical Materials Research Part A 6/1/2019 [PMID: 30698325]
• DT Dao, JT Vuong, L Anez-Busti, A Pan, PD Mitchell, GL Fell, MA Baker, DR Bielenberg, M Puder Intranasal delivery of VEGF enhances compensatory lung growth in mice PLoS ONE, 2018-06-07;13(6):e0198700. 2018-06-07 [PMID: 29879188] (Western Blot, Mouse)
• BE Hosford, S Rowley, JP Liska, SC Danzer Ablation of peri-insult generated granule cells after epilepsy onset halts disease progression Sci Rep, 2017-12-21;7(1):18015. 2017-12-21 [PMID: 29269775] (IHC-Fr, Mouse)
• Steve C Danzer Ablation of Newly Generated Hippocampal Granule Cells Has Disease-Modifying Effects in Epilepsy J. Neurosci., 2016-10-26;36(43):11013-11023. 2016-10-26 [PMID: 27798182] (IHC, Mouse)
• Li D, Wang A, Liu X, Meisgen F, Grunler J, Botusan I, Narayanan S, Erikci E, Li X, Blomqvist L, DU L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina S, Stahle M, Landen N MicroRNA-132 enhances transition from inflammation to proliferation during wound healing. J Clin Invest, 2015-06-29;125(8):3008-26. 2015-06-29 [PMID: 26121747] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: HBEGF
• Uniprot:
  • Q06186()