Recombinant Human HB-EGF Protein

SDS-Page: Human HB-EGF Protein [NBP2-34920]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human HB-EGF Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 86 amino acids corresponding to HB-EGF Source: E. coli

  Uniprot ID: Q99075

  Amino Acid Sequence: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL

• Details of Functionality: HB-EGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HBEGF
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of HB-EGF as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 9.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 10 mM PBS, 500 mM NaCl, pH 7.4.
• Preservative: 5% Trehalose
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/mL.

Alternate Names for Recombinant Human HB-EGF Protein

• diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor)
• Dtr
• DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor)
• Dts
• DTSF
• HBEGF
• HB-EGF
• Hegfl
• heparin-binding EGF-like growth factor
• heparin-binding epidermal growth factor
• proheparin-binding EGF-like growth factor

Background

Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation, wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Human HB-EGF shares about 73 % and 76 % a.a. sequence identity with murine and rat HB-EGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for HB-EGF Recombinant Protein (NBP2-34920)(1)

Publication using NBP2-34920

• He JH, Liu RP, Peng YM et al. Differential and paradoxical roles of new-generation antidepressants in primary astrocytic inflammation Journal of Neuroinflammation 2021-02-18 [PMID: 33602262]

Bioinformatics

• Gene Symbol: HBEGF
• Uniprot:
  • Q99075()
  • Q99075()
  • Q99075()