HCN3 Antibody

Western Blot: HCN3 Antibody [NBP1-68869] - NBP1-68869 (1ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Hu, Mu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HCN3 Antibody Summary

• Immunogen: Peptide with sequence C-RGVRGRAPSTGAQ corresponding to internal region according to NP_065948.1.
• Epitope: C-RGVRGRAPSTGAQ
• Predicted Species: Human (100%), Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HCN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 85 kDa band observed in rat kidney lysates (calculated MW of 86.0 kDa band according to human NP_065948.1 and 86.8 kDa band according to rat NP_446137.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HCN3 Antibody

• HCN3
• hyperpolarization activated cyclic nucleotide-gated potassium channel 3
• KIAA1535
• KIAA1535MGC131493
• potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Background

HCN3 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. HCN3 has been detected in the brain, but with less expression than other HCN channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HCN3 Antibody (NBP1-68869)(1)

Publication using NBP1-68869

• Hofmann F, Biel M, Kaupp UB. International Union of Pharmacology. LI. Nomenclature and structure-function relationships of cyclic nucleotide-regulated channels. Pharmacol Rev 2005-12-01 [PMID: 16382102]

Bioinformatics

• Gene Symbol: HCN3
• Entrez:
  • 57657(Human)
  • 15168(Mouse)
• Uniprot:
  • NP_065948.1(Human)