HDAC5 Antibody

Western Blot: HDAC5 Antibody [NBP2-03989] - Aanalysis of HDAC5 (Ab-498) (Lanes 1 and 2) using NBP2-03989 in NIH/3T3 cells.

Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP2-03989] - Analysis of HDAC5 in paraffin-embedded human breast carcinoma tissue using this antibodies

Product Discontinued

HDAC5 Antibody Summary

• Immunogen: This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human HDAC5 around the phosphorylation site of serine 498 (T-Q-SP-S-P).
• Specificity: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 18292392)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HDAC5 Antibody

• Antigen NY-CO-9
• EC 3.5.1.98
• FLJ90614
• HD5
• Histone Deacetylase 5
• KIAA0600
• NY-CO-9

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC5
• Uniprot:
  • NP_001015053(Human)