Human HDL ELISA Kit (Colorimetric)

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ELISA: Human HDL ELISA Kit (Colorimetric) [NBP2-68026] - Samples were spiked with high concentrations of Human HDL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Human HDL ELISA Kit (Colorimetric) [NBP2-68026] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.13 - 5000 ng/mL
Sensitivity
46.88 ng/mL

Order Details

Human HDL ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human HDL in samples. No significant cross-reactivity or interference between Human HDL and analogues was observed.
Standard Curve Range
78.13 - 5000 ng/mL
Sensitivity
46.88 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.56%
Intra-Assay
CV% < 4.84%
Spike Recovery
84-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human HDL ELISA Kit (Colorimetric)

  • APOA1/APOC3 fusion gene
  • Apolipoprotein A I precursor
  • Apolipoprotein of high density lipoprotein
  • High Density Lipoprotein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Additional HDL Products

Array NBP2-68026

Research Areas for HDL ELISA Kit (NBP2-68026)

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Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. ...  Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP...  Read full blog post.

LXR Alpha, ABCA1 and Cholesterol Homeostasis
LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee...  Read full blog post.

SR-BI Antibodies: A Potential for Blocking Hepatitis C Uptake
Scavenger Receptor Class B Membrane 1, also known as SR-BI plays an important role in lipid metabolism. Its main function is to mediate transfer of cholesterol between the cell surface and high density lipoprotein (HDL). HDL acts as an extracellular d...  Read full blog post.

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