Helios Antibody (22F6) - BSA Free

Flow Cytometry: Helios Antibody (22F6) [NBP2-37723] - Staining of CD4+ human peripheral blood lymphocytes using anti-Helios (clone 22F6) PE-CyTM7.

Flow Cytometry: Helios Antibody (22F6) [NBP2-37723] - Human peripheral blood lymphocytes were stained with anti-CD4 Pacific BlueTM, fixed and permeabilized with Treg Detection Kit and then intracellulary stained with anti-Helios PE (clone 22F6).

• Reactivity: Hu, Mu
• Applications: Flow, CyTOF-ready
• Clone: 22F6
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Helios Antibody (22F6) - BSA Free Summary

• Immunogen: Peptide coresponding to the amino acids 51-107 of Helios.
• Specificity: The armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: IKZF2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Helios Antibody (22F6) - BSA Free

• Helios
• IKAROS family zinc finger 2 (Helios)
• Ikaros family zinc finger protein 2
• IKZF2
• MGC34330
• subfamily 1A, 2 (Helios)
• zinc finger DNA binding protein Helios
• zinc finger protein Helios
• ZNF1A2
• ZNFN1A2

Background

HELIOS encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IKZF2