HELZ Antibody

Immunocytochemistry/Immunofluorescence: HELZ Antibody [NBP1-84253] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane & actin filaments.

Immunohistochemistry-Paraffin: HELZ Antibody [NBP1-84253] - Staining of human skin shows strong nucleolar positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: HELZ Antibody [NBP1-84253] - Staining of human cerebellum shows strong nucleolar positivity in Purkinje cells.

Immunohistochemistry-Paraffin: HELZ Antibody [NBP1-84253] - Staining of human cerebral cortex shows strong nucleolar positivity in neurons.

Immunohistochemistry-Paraffin: HELZ Antibody [NBP1-84253] - Staining of human kidney shows moderate nucleolar positivity in cells in tubules.

Product Discontinued

HELZ Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TQVSQEVPENCQEWIGGKMAQNGLDHYVYKVGIAFNTEIFGTFRQTIVFDFGLEPVLMQRVMIDAASTEDLEYLMHAKQQLVTTAKRWDSSSKTIIDFEPNETTDLEKSLLIRYQIPLSADQLFTQSVLDKSLTKSNYQSRL
• Predicted Species: Mouse (97%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HELZ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HELZ Antibody

• DHRC
• DKFZp586G1924
• Down-regulated in human cancers protein
• DRHC
• EC 3.6.4.-
• helicase with zinc finger
• HUMORF5helicase with zinc finger domain
• KIAA0054MGC163454
• probable helicase with zinc finger domain

Background

HELZ is a member of the superfamily I class of RNA helicases. RNA helicases alter the conformation of RNA by unwinding double-stranded regions, thereby altering the biologic activity of the RNA molecule and regulating access to other proteins (Wagner et al., 1999 [PubMed 10471385]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HELZ