Human Hepatitis A Virus Antibody (IgG) ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum

Human Hepatitis A Virus Antibody (IgG) ELISA Kit (Colorimetric) Summary

• Specificity: This assay has high sensitivity and excellent specificity for detection of human anti-HAV antibody (IgG). No significant cross-reactivity or interference between human anti-HAV antibody (IgG) and analogues was observed.
• Assay Type: Indirect ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Background

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HAV antigen. Samples are pipetted into the wells with mouse anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibody specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-HAV antibody (IgG) bound in the intial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.