Hepatocyte Specific Antigen Antibody (HepPar1)

Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272] - Formalin-fixed, paraffin-embedded human Liver stained with Hepatocyte Specific Antigen Antibody (HepPar1).

Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272] - Human Hepatocellular Carcinoma stained with HepPar-1 Monoclonal Antibody (HepPar1).

• Reactivity: Hu, Mu, Ca
• Applications: WB, ICC/IF, IHC, ICC/IF
• Clone: HepPar1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Hepatocyte Specific Antigen Antibody (HepPar1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08970)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Extract of a formalin-fixed, rejected-allograft of a human liver
• Localization: Finely granular cytoplasmic
• Marker: Hepatocellular Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25-0.5 ug/ml
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Use in Western Blot reported in scientific literature (PMID:31998631) .

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27542264).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Hepatocyte Specific Antigen Antibody (HepPar1)

• Hepatocyte
• HSA

Background

HSA (Human Serum Albumin) is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. It labels an antigen in the mitochondrial fraction of the liver homogenates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hepatocyte Specific Antigen Antibody (NBP2-45272)(9)

Publications using NBP2-45272

• Yanjing Zhu, Shijie Tang, Qiuyue Yuan, Jing Fu, Juan He, Zhuang Liu, Xiaofang Zhao, Yunguang Li, Yan Zhao, Yani Zhang, Xiaoyu Zhang, Yangqianwen Zhang, Yiqin Zhu, Wenwen Wang, Bo Zheng, Rui Wu, Tong Wu, Shuai Yang, Xinyao Qiu, Siyun Shen, Ji Hu, Luonan Chen, Yong Wang, Hongyang Wang, Dong Gao, Lei Chen Integrated characterization of hepatobiliary tumor organoids provides a potential landscape of pharmacogenomic interactions. Cell reports. Medicine 2024-02-23 [PMID: 38278146]
• Ahmadi AR, Song G, Gao T et al. Discovery and characterization of cross-reactive intrahepatic antibodies in severe alcoholic hepatitis bioRxiv : the preprint server for biology 2023-02-23 [PMID: 36865259] (MI, Human)
  
  Details:
  Used in multiplex immunofluorescence staining
• Imamura, Y;Hiyama, A;Miyazaki, H;Yamanaka, T;Nukina, N; Amyloids facilitate DNA transfection in vivo Neuroscience research [PMID: 35283247] (IF/IHC, Mouse)
• Rebecca Marcus, Sammy Ferri-Borgogno, Abdel Hosein, Wai Chin Foo, Bidyut Ghosh, Jun Zhao, Kimal Rajapakshe, James Brugarolas, Anirban Maitra, Sonal Gupta, Arndt Vogel Oncogenic KRAS Requires Complete Loss of BAP1 Function for Development of Murine Intrahepatic Cholangiocarcinoma Cancers 2021-11-15 [PMID: 34830866]
• Xu M, Zhou F, Ahmed O Et al. Dual Lactate Clearance in the Viability Assessment of Livers Donated After Circulatory Death With Ex Situ Normothermic Machine Perfusion Transplantation direct 2021-12-01 [PMID: 34805491] (ICC/IF, Human)
• Zhao Y, Li ZX, Zhu YJ et al. Single-Cell Transcriptome Analysis Uncovers Intratumoral Heterogeneity and Underlying Mechanisms for Drug Resistance in Hepatobiliary Tumor Organoids Science Advances 2021-06-08 [PMID: 34105295] (IF/IHC, Human)
• Qiu M, Glass Z, Chen J et al. Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3 Proceedings of the National Academy of Sciences of the United States of America 2021-03-09 [PMID: 33649229]
• Cai S, Bi Z, Bai Y et al. Glycyrrhizic Acid-Induced Differentiation Repressed Stemness in Hepatocellular Carcinoma by Targeting c-Jun N-Terminal Kinase 1 Front Oncol 2019-01-09 [PMID: 31998631] (WB, IHC-P, Mouse, Human)
• Zhang R, Guo H, Xu J et al. Activated platelets inhibit hepatocellular carcinoma cell differentiation and promote tumor progression via platelet-tumor cell binding. Oncotarget. 2016-09-13 [PMID: 27542264] (IF/IHC, Mouse)

Review for Hepatocyte Specific Antigen Antibody (NBP2-45272) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: and human, Human, Mouse.

reviewed by: Junhua Yang WB and human, Human, Mouse 12/01/2016

Summary

• Application: Western Blot
• Sample Tested: human liver cell,Liver tissue
• Species: and human, Human, Mouse
• Lot: MSM4

Comments

• Comments: HepPar1 Ab: Novus# NBP2-45272, 1:500 3%milk.
  Goat anti mouse 1:4000, size 165kd
  Sample: Human liver tissue