Recombinant Human HGF His Protein

SDS-Page: Recombinant Human HGF Protein [NBP2-51730] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human HGF His Protein Summary

• Description: A denatured recombinant protein with a His-tag corresponding to the amino acids 32-285 of Human HGF
  
  

  Source: E.coli

  Amino Acid Sequence: MQRKRRNTIH EFKKSAKTTL IKIDPALKIK TKKVNTADQC ANRCTRNKGL PFTCKAFVFD KARKQCLWFP FNSMSSGVKK EFGHEFDLYE NKDYIRNCII GKGRSYKGTV SITKSGIKCQ PWSSMIPHEH SYRGKDLQEN YCRNPRGEEG GPWCFTSNPE VRYEVCDIPQ CSEVECMTCN GESYRGLMDH TESGKICQRW DHQTPHRHKF LPERYPDKGF DDNYCRNPDG QPRPWCYTLD PHTRWEYCAI KTCET

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HGF
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 29.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human HGF His Protein

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for HGF Recombinant Protein (NBP2-51730)(2)

Publications using NBP2-51730

• Stout AJ Strategies for Enhanced Cultured Meat Products and Processes Thesis 2023-01-01
• Andrew J. Stout, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John S. K. Yuen, David L. Kaplan Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat Communications Biology 2022-06-02 [PMID: 35654948]

Bioinformatics

• Gene Symbol: HGF