HIF-3 alpha Antibody

Western Blot: HIF-3 alpha Antibody [NB100-2287] - Analysis of HIF-3 alpha in Jurkat cell lysate.

Product Discontinued

HIF-3 alpha Antibody Summary

• Immunogen: Bacterially expressed mouse HIF-3 alpha (N-terminus). [UniProt# Q0VBL6]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIF3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:200
• Application Notes: This HIF-3 alpha antibody is useful for Western blot, where a band is observed ~70 kDa.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 CoCl2 treated/Untreated Cell Lysate
  L929 CoCl2 treated/Untreated Cell Lysate
  Raw 264.7 CoCl2 treated/Untreated Cell Lysate
  Raw 264.7 Hypoxic / Normoxic Cell Lysate
  L929 Hypoxic / Normoxic Cell Lysate
  NIH 3T3 Hypoxic / Normoxic Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HIF-3 alpha Antibody

• Basic-helix-loop-helix-PAS protein MOP7
• BHLHe17
• bHLHe17HIF-3A2
• Class E basic helix-loop-helix protein 17
• HIF-3 alpha
• HIF3A
• HIF-3A4
• HIF-3-alpha
• HIF3-alpha
• hypoxia inducible factor 3, alpha subunit
• hypoxia-inducible factor 3-alpha
• hypoxia-inducible factor-3 alpha 4
• Inhibitory PAS domain protein
• IPAS
• IPASHIF3-alpha-1
• Member of PAS protein 7
• MOP7
• MOP7PAS domain-containing protein 7
• PASD7
• PASD7HIF-3A

Background

Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIF-3 alpha Antibody (NB100-2287)(3)

Publications using NB100-2287

• Jackson IL, Zhang X, Hadley C et al. Temporal expression of hypoxia-regulated genes is associated with early changes in redox status in irradiated lung Free Radic Biol Med 2012-04-25 [PMID: 22588005] (WB, Mouse)
• Tam, KKY. The role of hypoxia inducible factors in regulating ovarian function. Thesis (PhD) University of Adelaide, School of Paediatrics and Reproductive Health 2010-01-01 (WB, Mouse)
• Ouyang L, Sun Y, Lv D et al. miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3 alpha in bone iScience 2022-01-01 [PMID: 35005549] (WB, Mouse)

FAQs for HIF-3 alpha Antibody (NB100-2287). (Showing 1 - 1 of 1 FAQs).

1. The customer wants to know the immunogen sequence.
   • NB100-2287 was made to a partial recombinant protein that is found between amino acids 1-275.

Bioinformatics

• Gene Symbol: HIF3A
• Uniprot:
  • Q9Y2N7(Human)
  • Q0VBL6(Mouse)
  • Q0VBL6(Mouse)