High Efficiency Transfection reagent Transfection Reagent Kit

Product Discontinued

High Efficiency Transfection reagent Transfection Reagent Kit Summary

• Kit Type: Transfection Reagent Kit

Applications/Dilutions

• Application Notes: Use the following procedure to transfect DNA into eukaryotic cells in a 35 mm dish or a 6-well plate.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Prediluted antibody

Notes

Major Features:
Exceptional efficiency of transfection for a broad range of adherent cell types.
Especially good for embryonic stem (ES) cells and difficult-to-transfect primary cultures.
Equally efficient for suspension cultures of HEK293, and sf9, sf21 and High 5 insect cells.
Higher efficiency and lower toxicity than all commercially available transfection reagents currently on the market.
Transfection efficiency not affected by serum. Works equally well in serum-containing (up to 20% FBS) or serum-free media.
High levels of recombinant protein production at 24-72 hours after transfection.
Works very well for both single DNA transfection and multi-DNA co-transfection.
This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.

Background

This product is a lipid-based reagent designed and formulated with proprietary technology for transfecting DNA or siRNA into a variety of eukaryotic cell lines, including HEK293, CHO, COS, NIH3T3, MCF-7, PC12, RKO, C2C12 myoblasts, mouse embryonic stem cells (ES) and fibroblasts (MEF), mesenchymal stem cells, NCCIT, S2 (Schneider) Drosophila cells as well as primary cultures of human carcinoma, rat adrenal chromaffin cells, mouse and rat cortical neurons, hepatocytes, and etc. This transfection reagent also offers extremely high transfection efficiencies and minimal cytotoxicity for suspension cultures including HEK 293, mouse lymphocytes, and insect cells (sf9, sf21, High-Five). The reagent works very well in cultures with serum concentration range from 0 to 20% FBS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Review for High Efficiency Transfection reagent Kit (NBP1-30438) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Katherine Uyhazi Flow Mouse 07/22/2010

Summary

• Application: Flow Cytometry
• Sample Tested: Mouse Embryonic Stem Cells
• Species: Mouse