Fish Histamine ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1420
    • Availability
      Product Discontinued

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Fish Histamine ELISA Kit (Colorimetric) Summary

Assay Type
Competitive ELISA
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Histamine ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of histamine.

Reactivity Notes

Fish

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 96-well Microtiter plate (#S): Twelve strips of 8 detachable wells, coated withAnti-Histamine antibody.
  2. Calibrator: 0, 2.5, 5.0 and 10.0 µg/mL of histamine.
  3. Histamine-Alkaline Phosphatase conjugate (HTM-ALP) (#3)
  4. p-Nitrophenyl Phosphate (pNPP) substrate (#5). Ready to use.
  5. Stop Solution (#7): 3 N NaOH.
  6. Wash Buffer (10x PBS-Tween) (#6). Dilute 10 fold with distilled or deionized water to150 mL prior to use.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fish Histamine ELISA Kit (Colorimetric)

  • 2 (4 imidazolyl)ethylamine
  • 4 (2 aminoethyl) 1 3 diazole

Background

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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