Histamine ELISA Kit (Colorimetric)

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ELISA: Histamine ELISA Kit (Colorimetric) [NBP2-62860] - Samples were spiked with high concentrations of Histamine and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Histamine ELISA Kit (Colorimetric) [NBP2-62860] - Standard Curve Reference

Product Details

Summary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

Order Details

Histamine ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes HIS in samples. No significant cross-reactivity or interference between HIS and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Competitive-ELISA
Inter-Assay
CV% < 4.58%
Intra-Assay
CV% < 4.66%
Spike Recovery
87-105%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using
NBP2-62860 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Histamine ELISA Kit (Colorimetric)

  • 2 (4 imidazolyl)ethylamine
  • 4 (2 aminoethyl) 1 3 diazole

Background

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Histamine ELISA Kit (NBP2-62860)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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