Histone H3 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Histone H3 Antibody [NB100-747] - Human Prostate 5 ug/ml.

Immunohistochemistry-Paraffin: Histone H3 Antibody [NB100-747] - Human Prostate 5 ug/ml.

• Reactivity: Hu, All, Bv
• Applications: WB, ICC/IF, IHC, IP, Single-Cell Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Histone H3 Antibody - Azide and BSA Free Summary

• Immunogen: This Histone H3 antibody was raised against intact calf histone H3 at 15.4 kD, 136 amino acids, complexed with RNA.
• Localization: Nuclear
• Specificity: This Histone H3 antibody exhibits minimal cross reactivity with linker histone H1 and no cross-reactivity with histone core proteins H2A, H2B, or H4.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: H3C14
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:1000
  • Single Cell Western 100 ug/mL
  • Western Blot 1:300
• Application Notes: Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID: 30123962). SCW is validated using primary murine germ cells.
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Colon Whole Tissue Lysate (Adult Whole Tumor)	Loading...
  Human Tonsil Whole Tissue Lysate (Adult Whole Normal)	Loading...

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Histone H3 Antibody - Azide and BSA Free

• H3 histone, family 3A
• H3
• H3.3A
• H3.3B
• H3F2
• H3F3
• H3F3A
• H3F3B
• H3FM
• HIST2H3C
• Histone H3
• histone H3.3
• MGC87782
• MGC87783

Background

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone H3 Antibody (NB100-747)(6)

Publications using NB100-747

• Dell'Orso S, Juan AH, Ko KD et al. Single-cell analysis of adult skeletal muscle stem cells in homeostatic and regenerative conditions Development 2019-03-19 [PMID: 30890574] (WB, Human)
• Fernandez-Vega I, Diaz-Lucena D, Azkune Calle I et al. Sporadic Creutzfeldt-Jakob disease with glial PrPRes nuclear and perinuclear immunoreactivity Neuropathology 2018-08-19 [PMID: 30123962] (ICC/IF, Human)
• Kasemeier-Kulesa JC, Schnell S, Woolley T et al. Predicting neuroblastoma using developmental signals and a logic-based model Biophys. Chem. 2018-07-01 [PMID: 29734136] (Human)
• McLennan R, Bailey CM, Schumacher LJ et al. DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion. J. Cell Biol. 2017-08-15 [PMID: 28811280] (Human)
• Dart DA et al. Recruitment of the cell cycle checkpoint kinase ATR to chromatin during S-phase. J Biol Chem 279:16433-40. 2004-01-01 [PMID: 17468514] (WB)
• Capozzo AV, Burke DJ, Fox JW, Bergmann IE, La Torre JL, Grigera PR. Expression of foot and mouth disease virus non-structural polypeptide 3ABC induces histone H3 cleavage in BHK21 cells. Virus Res;90(1-2):91-9. 2002-12-01 [PMID: 12457965] (WB)

Bioinformatics

• Gene Symbol: H3C14