HIV-1 gp120 ELISA Kit (Colorimetric) Summary
Specificity |
Recognizes both recombinant and natural HIV-1 gp120. |
Standard Curve Range |
34.38-2200 pg/ml (example only; lot dependent) |
Sensitivity |
14.47 pg/ml |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 4.53 |
Intra-Assay |
CV% < 3.1 |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
env |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage is content dependent. |
Kit Components
Components
|
- 1. 96 well microplate coated with Capture Antibody
- 2. Detection Antibody conjugated to HRP
- 3. Standards
- 4. Wash Buffer Concentrate
- 5. Dilution Buffer Concentrate
- 6. Color Reagent A
- 7. Color Reagent B
- 8. Stop Solution
|
Alternate Names for HIV-1 gp120 ELISA Kit (Colorimetric)
Background
HIV-1 gp120 is a surface glycoprotein that enables viral attachment and entry into host cells. It is also involved in neurotoxicity and has been implicated in HIV-associated neurocognitive disorders. HIV-1 gp120 also induces apoptosis in neuronal cells by signaling mitochondrial-death proteins which may upregulate the death receptor, Fas. Because HIV gp120 is necessary for HIV-1 to enter CD4 cells, gp120 is one of the main targets of HIV vaccine research.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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