HLA-E Antibody (3D12HLA-E) [PE]

Flow Cytometry: HLA-E Antibody (3D12HLA-E) [PE] [NBP2-00277] - Staining of normal human peripheral blood cells with Anti-Human HLA-ABC FITC and Mouse IgG1 kappa Isotype Control PE (left) or Anti-Human HLA-E PE (right). Cells in the lymphocyte gate were used for analysis.

Product Discontinued

HLA-E Antibody (3D12HLA-E) [PE] Summary

• Immunogen: The immunogen for this antibody was HLAE.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-E
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: This 3D12 (HLA-E) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 ul (0.5 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 5 mg/ml
• Purity: Protein A or G purified

Alternate Names for HLA-E Antibody (3D12HLA-E) [PE]

• DKFZp686P19218
• EA1.2
• EA2.1
• HLA class I histocompatibility antigen, alpha chain E
• HLA class I histocompatibility antigen, E alpha chain
• HLA-6.2
• HLAE
• HLA-E
• lymphocyte antigen
• major histocompatibility complex, class I, E
• MHC class I antigen E
• MHC HLA-E alpha-1
• MHC HLA-E alpha-2.1
• MHC
• QA1

Background

The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-E