HMGCS2 Antibody (5T5T3)

Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696] - Western blot analysis of extracts of various cell lines, using HMGCS2 antibody (NBP3-15696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry-Paraffin: HMGCS2 Antibody (5T5T3) [NBP3-15696] - Immunohistochemistry of paraffin-embedded human liver using HMGCS2 Rabbit mAb (NBP3-15696) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696] - Western blot analysis of extracts of HepG2 cells, using HMGCS2 antibody (NBP3-15696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Product Discontinued

HMGCS2 Antibody (5T5T3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 409-508 of human HMGCS2 (P54868). AFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHRRKYARRPV
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HMGCS2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.05% BSA, 50% glycerol, pH7.3
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HMGCS2 Antibody (5T5T3)

• 3-hydroxy-3-methylglutaryl coenzyme A synthase
• 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial)
• 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial)
• EC 2.3.3.10
• HMG-CoA synthase
• hydroxymethylglutaryl-CoA synthase, mitochondrial
• mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase

Background

HMGCS2 is an enzyme that condenses acetyl CoA with acetoacetyl CoA to form HMG CoA, which is the substrate for HMG CoA reductase. Together with HMG CoA lyase, it is responsible for ketone body biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGCS2