HNF1 Antibody (HNF1A/2087)

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).

Protein Array: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HNF1 Antibody (HNF1A/2087). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: ELISA, IHC, MA
• Clone: HNF1A/2087
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HNF1 Antibody (HNF1A/2087) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79911)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (around aa 214-339) of human HNF1 protein (exact sequence is proprietary) (Uniprot: P20823)
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNF1A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA).
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for HNF1 Antibody (HNF1A/2087)

• HNF1 homeobox A
• IDDM20
• LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor
• MODY3
• TCF1hepatic
• Transcription factor 1

Background

HNF1 is a transcription factor required for the expression of several liver-specific genes. The protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNF1A
• Uniprot:
  • P20823(Human)